Fig 3

(A) The cell density of dlc⁺ ionocyte progenitors was reduced in embryos injected with both klf4 MO1 and MO2 (c) as compared to uninjected wild type (a) or control embryos injected with klf4 5mmMO2 (b) at bud stage. Cell densities of dlc⁺ ionocyte progenitors were quantified from the indicated area in wild-type embryos and embryos injected with klf4 5mmMO2 or both klf4 MO1 and MO2 (d). The density of dlc⁺ ionocyte progenitors was increased in klf4-overexpressing embryos (g) compared to LacZ-overexpressing (f) or uninjected wild-type (e) embryos. Cell density of dlc⁺ ionocyte progenitors was quantified in wild-type embryos and LacZ or klf4 mRNA-injected embryos (h). (B) The density of foxi3a⁺ ionocyte progenitors was reduced in embryos injected with both klf4 MO1 and MO2 (c), as compared to uninjected wild-type (a) or control embryos injected with klf4 5mmMO2 (b) at 5s stage. Cell densities of foxi3a⁺ ionocyte progenitors were quantified from the indicated area in wild-type embryos and embryos injected with klf4 5mmMO2 or both klf4 MO1 and MO2 (d). The density of foxi3a⁺ ionocyte progenitors was increased in klf4-overexpressing embryos (g), as compared to LacZ-overexpressing (f) or uninjected wild-type (e) embryos. Cell densities of foxi3a⁺ ionocyte progenitors were quantified in wild-type embryos and LacZ- or klf4-injected embryos (h). (C) The density of foxi3b⁺ ionocyte progenitors was reduced in embryos injected with both klf4 MO1 and MO2 (c) as compared to uninjected wild-type (a) or control embryos injected with klf4 5mmMO2 (b) at 5s stage. Cell density of foxi3b⁺ ionocyte progenitors was quantified from the indicated area in wild-type embryos and embryos injected with klf4 5mmMO2 or both klf4 MO1 and MO2 (d). The density of foxi3b⁺ ionocyte progenitors was increased in klf4-overexpressing embryos (g) compared to LacZ-overexpressing (f) or uninjected wild-type (e) embryos. Cell density of foxi3b⁺ ionocyte progenitors was quantified in wild-type embryos and LacZ or klf4 mRNA injected embryos (h). Embryos are shown in lateral view. Statistical significance was determined by Student’s t-test. *p < 0.05; ***p < 0.001. Scale bar, 100 μm. Error bars indicate standard error.