FIGURE

Figure 2

ID

ZDB-FIG-190723-1301

Publication

Marra et al., 2019 - Iroquois transcription factor irx2a is required for multiciliated and transporter cell fate decisions during zebrafish pronephros development

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Figure 2

irx2a restricts the PST and promotes the DL during pronephros segmentation. (A) Lateral view of WISH analysis with the PST marker trpm7 at the 28 ss. Black bars highlight the trpm7 expression domain in the pronephros. Scale bar is 50 μm. (B) Quantification of trpm7 length (μm). Each dot represents one nephron and data is presented +/− SEM. Statistical significance was determined with ANOVA. (C) WISH in 28 ss embryos with the DL marker slc12a3 shown in a lateral view. Black bars denote slc12a3 expression. (D) Quantification of slc12a3 domain length (μm), where each dot represents one nephron. Data is presented +/− SEM and statistical significance was determined by ANOVA. WISH – whole mount in situ hybridization, ss – somite stage, PST – proximal straight tubule, DL – distal late tubule.

Expression Data

Genes:	slc12a3 trpm7
Fish:	TU TU + MO2-irx2a
Knockdown Reagent:	MO2-irx2a
Anatomical Terms:	pronephric distal late tubule pronephric proximal straight tubule
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + MO2-irx2a
Knockdown Reagent:	MO2-irx2a
Observed In:	pronephric distal late tubule pronephric distal late tubule pronephric nephron tubule epithelial cell differentiation pronephric proximal straight tubule pronephric proximal straight tubule pronephric nephron tubule epithelial cell differentiation
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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