FIGURE

Fig. S2

ID

ZDB-FIG-190716-6

Publication

Han et al., 2019 - Vitamin D Stimulates Cardiomyocyte Proliferation and Controls Organ Size and Regeneration in Zebrafish

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Fig. S2

cyp24a1mGFP Reporter Fish Indicate Vitamin D Activity (Related to

Figure 2)

(A) mGFP fluorescence in 24 hpf and 48 hpf cyp24a1mGFP embryos. Scale bar, 500 μm.

(B) Lateral view of a cyp24a1mGFP reporter knock-in at 3 dpf following treatment with

vehicle or Alfa for 24 hours. Arrowheads indicate cardiac mGFP fluorescence. Scale bar,

500 μm.

hearts after vehicle or Alfa treatment for 24 hours. Scale bar, 50 μm.

(D) Maximum intensity projection images of dissected 4 dpf fabp10a:nls-mCherry;

cyp24a1mGFP livers (to mark hepatocytes) after vehicle or Alfa treatment for 24 hours.

Scale bar, 100 μm.

(E) Representative 4 dpf wild-type embryo from Figure 2B indicating section plane of

Figures F’ to F’’’’ with dashed lines.

(F) Maximum projections of 10 μm confocal sections at indicated planes of 4 dpf

krtt1c19e:H2A-mCherry; cyp24a1mGFP (to mark basal epithelial cells) embryos treated

with vehicle or Alfa. Scale bars, 100 μm for F’-F’’’ and 20 μm for F’’’’.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 48(6), Han, Y., Chen, A., Umansky, K.B., Oonk, K.A., Choi, W.Y., Dickson, A.L., Ou, J., Cigliola, V., Yifa, O., Cao, J., Tornini, V.A., Cox, B.D., Tzahor, E., Poss, K.D., Vitamin D Stimulates Cardiomyocyte Proliferation and Controls Organ Size and Regeneration in Zebrafish, 853-863.e5, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell