Fig. S2

Fig. S2

cyp24a1mGFP Reporter Fish Indicate Vitamin D Activity (Related to

Figure 2)

(A) mGFP fluorescence in 24 hpf and 48 hpf cyp24a1mGFP embryos. Scale bar, 500 μm.

(B) Lateral view of a cyp24a1mGFP reporter knock-in at 3 dpf following treatment with

vehicle or Alfa for 24 hours. Arrowheads indicate cardiac mGFP fluorescence. Scale bar,

500 μm.

(C) Maximum intensity projection images of dissected 4 dpf cmlc2:dsRed;cyp24a1mGFP

hearts after vehicle or Alfa treatment for 24 hours. Scale bar, 50 μm.

(D) Maximum intensity projection images of dissected 4 dpf fabp10a:nls-mCherry;

cyp24a1mGFP livers (to mark hepatocytes) after vehicle or Alfa treatment for 24 hours.

Scale bar, 100 μm.

(E) Representative 4 dpf wild-type embryo from Figure 2B indicating section plane of

Figures F’ to F’’’’ with dashed lines.

(F) Maximum projections of 10 μm confocal sections at indicated planes of 4 dpf

krtt1c19e:H2A-mCherry; cyp24a1mGFP (to mark basal epithelial cells) embryos treated

with vehicle or Alfa. Scale bars, 100 μm for F’-F’’’ and 20 μm for F’’’’.