Fig. 2

Vitamin D Signaling Enhances Organismal Size

(A) MIP images and quantification of H3P staining of 3 dpf embryos. hpt, hours post-treatment; H3P, phospho-Histone H3. Mean ± SD, Mann-Whitney test and Student's t test. ^∗∗∗∗p < 0.0001. n = 11–13. Scale bar, 500 μm.

(B) Example and quantification of 4 dpf zebrafish growth. Mean ± SD, Mann-Whitney test and Student’s t test. ^∗∗p < 0.01; ^∗∗∗p < 0.001. n = 12-14. Scale bar, 500 μm.

(C) Growth and rescue of 2 mpf juvenile wild-type (WT) and ubb:cyp24a1 zebrafish. Scale bar, 4 mm.

(D) Quantification of zebrafish length before and after treatment. Mean ± SD. n = 14–15.

(E) mGFP and TagBFP expression in 4 dpf embryos treated with vehicle or 10 nM Alfa. Scale bar, 500 μm.

(F) Quantified body length of two independent ubb:cyp24a1 lines and WT siblings at 4 dpf. Mean ± SD, Mann-Whitney test. ^∗p < 0.05. n = 14–23.

(G) cyp24a1^mGFP expression in 1 μM Alfa-treated embryos.

(H) Quantification of relative growth of 4 dpf embryos treated with vehicle or Alfa for 24 h. Mean ± SD, Student’s t test. n = 13.

(I) Representative images and body length quantification of 6 mpf zebrafish. mpf, months post-fertilization. Mean ± SD, Student's t test. n = 7. Scale bar, 5 mm.

(J) mGFP and TagBFP expression of 3 dpf embryos after a single heat-shock and 24-h drug treatment. Scale bar, 500 μm.

(K) Quantified relative growth of 4 dpf embryos after a heat shock and 24-h drug treatment. Mean ± SD, Student’s t test. n = 10.