FIGURE

Fig. S11

ID

ZDB-FIG-181206-12

Publication

Ahlberg et al., 2018 - Rare truncating variants in the sarcomeric protein titin associate with familial and early-onset atrial fibrillation

Other Figures

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Fig. S11

Defective z-discs in a zebrafish titin truncated homozygous mutant

Top panel) Isolated hearts form 72 hours post fertilization old zebrafish stained for alpha-actinin, an important component of the Z-disc, revealed well-defined z-discs in both the atria (right chamber) and ventricle (left chamber) in the WT siblings. A close up of the atria (right) show well-defined z-discs (red arrows). Bottom panel) Compared to the observations made in the isolated hearts from the WT sibling embryos, the homozogous mutant embryos showed a no well-defined z-discs, and an aberrant alpha-actinin expression in the atria (indicated by the red arrows in the bottom right image) and the ventricle indicating a compromised sarcomere architecture. Scale bars = 20μm. Please see supplementary Figure 9-10 for complete z-stacks.

Expression Data

Antibody:	Ab1-actn
Fish:	ttn.2^sfc9/sfc9
Anatomical Term:	atrium
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ttn.2^sfc9/sfc9
Observed In:	atrium atrium sarcomere atrium Z disc
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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