FIGURE

Fig. 1

ID

ZDB-FIG-181101-7

Publication

Fernandez et al., 2018 - RES complex is associated with intron definition and required for zebrafish early embryogenesis

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Fig. 1

RES complex is essential for early vertebrate development.

(A) Schematic model of the RES complex adapted from Brooks et al. [8]. Rbmx2 (light blue) is the core subunit with an RRM-domain structure. Bud13 (orange) and Snip1 (pink) interact with Rbmx2 (light blue). (B) In situ hybridization showing spatial and temporal expression of RES complex members. (C-E) Gene models of the mutant allele generated using CRISPR-Cas9-nanos. (C) bud13, 7 nt deletion in exon 6 generated a premature stop codon. (D) rbmx2, 16 nt deletion removed exon-intron boundary at exon 2 (exon capital letter, intron lower letter). (E) snip1, 11 nt deletion in exon 1 generated a premature stop codon. f-g) Lateral view of RES complex mutant embryos, their corresponding WT sibling and mutants injected with the cognate mRNA. (F) bud13 mutant at 32 hpf (scale bar: 0.35mm) and at 48 hpf (scale bar: 0.5mm). (G, H) rbmx2 and snip1 mutant at 48 hpf respectively. WT: represent phenotypically wild type sibling from the same mutant fish line.

Expression Data

Genes:	bud13 rbmx2 snip1
Fish:	WT
Anatomical Terms:	blastodisc central nervous system
Stage Range:	High to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	bud13^ya103/ya103 rbmx2^ya102/ya102 snip1^ya104/ya104
Observed In:	brain central nervous system cell death
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

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