FIGURE

Fig. 2

ID

ZDB-FIG-181101-8

Publication

Fernandez et al., 2018 - RES complex is associated with intron definition and required for zebrafish early embryogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

RES complex is required during zebrafish brain development.

(A) Acridine orange (ao) staining of zebrafish mutant embryos for bud13 (30–32 hpf), rbmx2 and snip1 (48 hpf). Mutants show a marked degree of cells with nuclear uptake of ao compared to WT sibling most predominantly in the head. WT: represent phenotypically wild type sibling from the same mutant fish line. (B) Maximum intensity projections of individual and merged channels (GFP and dsRed) of 3D confocal images of bud13^Δ7/Δ7 and their wild-type siblings (scale bar 20 μm) in transgenic lines that label GABAergic neurons and precursors (Tg[dlx6a-1.4kbdlx5a/ dlx6a:GFP]) and glutamatergic neurons (Tg[vglut:DsRed]). WT: represent phenotypically wild type sibling from the same mutant fish line. (C) Total number of dlx5a/6a:GFP+ cells (GABAergic neurons and precursors) and vglut:DsRed+ cells (glutamatergic neurons) in the forebrain of the bud13^Δ7/Δ7 (n = 3) and WT sibling (n = 4) were quantified. *** vglut: P = 2 x 10⁻⁴; ***dlx5a/6a: P = 3 x 10⁻⁴ (one-way ANOVA).

Expression Data

Genes:	DsRed GFP
Fish:	nns9Tg; ot1Tg bud13^ya103/ya103; nns9Tg; ot1Tg
Anatomical Terms:	GABAergic neuron glutamatergic neuron
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	bud13^ya103/ya103 rbmx2^ya102/ya102 snip1^ya104/ya104 bud13^ya103/ya103; nns9Tg; ot1Tg
Observed In:	central nervous system cell death GABAergic neuron glutamatergic neuron
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.