FIGURE

Fig. 5

ID

ZDB-FIG-181019-6

Publication

Sternberg et al., 2018 - Pkd2l1 is required for mechanoception in cerebrospinal fluid-contacting neurons and maintenance of spine curvature

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Fig. 5

Pkd2l1 is necessary to maintain a straight spine. a Representative pkd2l1^+/+ and sibling pkd21^?/? adult zebrafish at adult stages. Scale: 1?cm. b Body curvature traces from pkd2l1^+/+ (black) and pkd2l1^?/? (blue) adults at 12 months (n?=?11 pkd21^+/+, n?=?11 pkd2l1^?/?). c Quantification of body axis curvature. Each point represents one fish. Error bars represent s.e.m. (5 months: n?=?14 pkd2l1^+/+, n?=?13 pkd2l1^?/?, p?=?0.02; 12 months: n?=?11 pkd2l1^+/+, n?=?11 pkd2l1^?/?, p?=?1.42?�?10^?4; 19 months: n?=?7 pkd2l1^+/+, n?=?11 pkd2l1^?/?, p?=?1.5?�?10^?3; two-sample t test). d Alizarin red staining of bones of an adult pkd2l1^+/+ fish at 20 months of age, showing little to no spinal kyphosis. Scale: 5?mm. e High-magnification image of the precaudal region of the pkd2l1^+/+ spine. Dotted lines indicate vectors used to calculate the Cobb angle. Scale: 1?mm. f Alizarin red staining of an adult pkd2l1^?/? fish. Note the kyphosis in the precaudal region. Scale: 5?mm. g High-magnification image reveals pronounced kyphosis, no torsion is evident (see Supplementary Table 2 for Cobb angles). Scale: 1?mm

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pkd2l1^icm02/icm02 (AB/TL)
Observed In:	vertebral column vertebral column skeletal system development
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.