FIGURE

Fig. 5

ID

ZDB-FIG-181019-6

Publication

Sternberg et al., 2018 - Pkd2l1 is required for mechanoception in cerebrospinal fluid-contacting neurons and maintenance of spine curvature

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Fig. 5

Pkd2l1 is necessary to maintain a straight spine. a Representative pkd2l1^+/+ and sibling pkd21^−/− adult zebrafish at adult stages. Scale: 1 cm. b Body curvature traces from pkd2l1^+/+ (black) and pkd2l1^−/− (blue) adults at 12 months (n = 11 pkd21^+/+, n = 11 pkd2l1^−/−). c Quantification of body axis curvature. Each point represents one fish. Error bars represent s.e.m. (5 months: n = 14 pkd2l1^+/+, n = 13 pkd2l1^−/−, p = 0.02; 12 months: n = 11 pkd2l1^+/+, n = 11 pkd2l1^−/−, p = 1.42 × 10^‒4; 19 months: n = 7 pkd2l1^+/+, n = 11 pkd2l1^−/−, p = 1.5 × 10^‒3; two-sample t test). d Alizarin red staining of bones of an adult pkd2l1^+/+ fish at 20 months of age, showing little to no spinal kyphosis. Scale: 5 mm. e High-magnification image of the precaudal region of the pkd2l1^+/+ spine. Dotted lines indicate vectors used to calculate the Cobb angle. Scale: 1 mm. f Alizarin red staining of an adult pkd2l1^−/− fish. Note the kyphosis in the precaudal region. Scale: 5 mm. g High-magnification image reveals pronounced kyphosis, no torsion is evident (see Supplementary Table 2 for Cobb angles). Scale: 1 mm

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pkd2l1^icm02/icm02 (AB/TL)
Observed In:	vertebral column vertebral column skeletal system development
Stage:	Adult

Phenotype Detail

Acknowledgments

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