FIGURE

Fig. S4

ID

ZDB-FIG-181019-10

Publication

Sternberg et al., 2018 - Pkd2l1 is required for mechanoception in cerebrospinal fluid-contacting neurons and maintenance of spine curvature

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Fig. S4

The cfap298 mutation does not abolish sensory responses to muscle contraction in 1 dpf zebrafish.

(a) Top: Images of Tg(pkd2l1:GCaMP5G) in cfap298+/+, cfap298tm304/+, cfap298tm304/tm304. Scale: 50 μm. Bottom: Sample calcium imaging traces of CSF-cNs at 1 dpf during active muscle contractions. Gray bars indicate periods of contraction when the cell moves out of the imaging plane. Scale: 10 s, 100% ΔF/F. (b) Responses to muscle contractions in individual cells for each genotype (cfap298+/+ n = 7 fish, 140 cells; cfap298tm304/+ n = 12 fish, 275 cells; cfap298tm304/tm304 n = 20 fish, 430 cells). (c) Cumulative distribution plot of the responses for all genotypes shows a reduced response of CSF-cNs to spontaneous contraction in cfap298tm304/tm304 embryos (linear mixed models, p = 5 x 10-4).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.