FIGURE

Fig. 1

ID

ZDB-FIG-181019-3

Publication

Sternberg et al., 2018 - Pkd2l1 is required for mechanoception in cerebrospinal fluid-contacting neurons and maintenance of spine curvature

Other Figures

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Fig. 1

Bidirectional flow of cerebrospinal fluid in the central canal of the spinal cord. a Single frames from time lapses of 24 h post fertilization (hpf) Tg(β-actin:Arl13B-GFP) embryos expressing GFP in cilia. Images taken from progressively dorsal focal planes. Scale: 25 µm. b Quantification of ciliary motion from time lapses; ventral planes exhibit more ciliary motion than dorsal planes (one-way ANOVA F = 12.2, p = 5.92 × 10^‒7, see Methods for motion index quantification) SC spinal cord, CC central canal. c Lateral view of the central canal in transmitted light (top) and filled with fluorescent beads (bottom). Scale: 20 µm. d Schematic of horizontal slices taken for kymograph analysis for velocities of beads or endogenous particles. e Representative kymographs of beads in the central canal. Analyzed trajectories are in red (dorsal) or blue (ventral). f Imaging setup for full-field optical coherence tomography (FF-OCT). g Lateral view of the central canal showing endogenous particles obtained from FF-OCT. Scale: 20 µm. h Representative kymographs of endogenous particles in the central canal. Analyzed trajectories are in red (dorsal) or blue (ventral). i Velocities of exogenous particles (beads) and endogenous particles. Beads: 307 trajectories from n = 7 embryos; endogenous particles: 452 trajectories from n = 7 embryos. Error bars represent s.e.m. D dorsal, V ventral, R rostral, C caudal

Expression Data

Gene:	GFP
Fish:	hsc5Tg (AB/TL)
Anatomical Terms:	central canal mechanoreceptor cell central canal motile cilium spinal cord motile cilium
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hsc5Tg (AB/TL)
Observed In:	spinal cord cerebrospinal fluid circulation spinal cord epithelial cilium movement involved in extracellular fluid movement
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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