Fig. S2

No signs of white matter pathology in csf1r mutant zebrafish. Related to Fig. 2.

A Representative images of 5-month-old WT (n=3), csf1ra^-/- (n=3), csf1ra^-/-;b^+/- (n=3) and csf1r^DM (n=3) zebrafish brain sections stained with an antibody against ClaudinK (myelin). B Electron microscopy was performed on the dissected brains of WT (n=3), csf1ra^-/-;b^+/- (n=3) and csf1r^DM (n=3) zebrafish (5 mpf). Representative images of myelin in WT, csf1ra^-/-;b^+/- and csf1r^DM. C. Morphometric analysis of axons in brains of WT and csf1ra^-/-;b^+/- and csf1r^DM mutant zebrafish, showing g-ratio (axon diameter/myelinated fiber diameter) at 5 months of age. Mean gratio’s presented as mean ± SEM were 0.74 +/- 0.01 for controls, 0.76+/- 0.01 for csf1ra^-/-;b^+/- and 0.77 +/- 0.0 for csf1r^DM and did not differ between genotypes according to t-test (p = 0.22, 0.12 and 0.34 respectively). D Representative images of Sox10 (oligodendrocyte lineage cells) antibody labeling in deep brain regions of zebrafish. Adult zebrafish, aged 5 mpf, were used in A, B and C and aged 15 mpf in D. B Scale bars represent 200 μm (A), 1 μm (B), 40 μm (D). Each group in (C.) consisted of two to three zebrafish brains, and contained at least 240 myelinated fibers per genotype.