FIGURE

Fig. 2

ID

ZDB-FIG-180911-40

Publication

Oosterhof et al., 2018 - Colony-Stimulating Factor 1 Receptor (CSF1R) Regulates Microglia Density and Distribution, but Not Microglia Differentiation In�Vivo

Other Figures

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Fig. 2

Altered Microglia Distribution and Numbers in the Adult csf1r Mutant Brain

(A) Representative images of microglia in WT, csf1ra^?/?, csf1ra^?/?;b^+/?, and csf1r^DM brain sections from zebrafish aged 5 months post fertilization (mpf), stained with antibody against L-plastin (n = 3 zebrafish/group).

(B) Microglia in (a) the ventral part of the optic tectum and (b) the dorsolateral part of the optic tectum of WT, csf1ra^?/?, csf1ra^?/?;b^+/?, and csf1r^DM.

(C) Representative images of microglia in WT, csf1ra^?/?, and csf1rb^?/? brain sections from 15 mpf zebrafish stained with antibody against L-plastin (n = 3 zebrafish/group).

Microglia were quantified in 3 areas (2.5 � 10⁶ ?m³) per brain region per animal. Error bars represent SD. ^?p < 0.05, ^??p < 0.01, ^???p < 0.001 (one-way ANOVA, Bonferroni multiple testing correction). Scale bars in (A) and (B), top, indicate 200 ?m; in (A) and (B), bottom, and in (C), scale bars indicate 50 ?m.

Expression Data

Antibody:	Ab2-lcp1
Fish:	AB csf1ra^j4e1/j4e1 csf1ra^j4e1/j4e1; csf1rb^re01/+ csf1ra^j4e1/j4e1; csf1rb^re01/re01
Anatomical Terms:	brain microglial cell optic tectum microglial cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	csf1ra^j4e1/j4e1 csf1rb^re01/re01 csf1ra^j4e1/j4e1; csf1rb^re01/+ csf1ra^j4e1/j4e1; csf1rb^re01/re01
Observed In:	brain microglial cell microglial cell optic tectum dorso-lateral region optic tectum microglial cell optic tectum ventral region
Stage:	Adult

Phenotype Detail

Acknowledgments

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