FIGURE

Fig. 6

ID

ZDB-FIG-180911-46

Publication

Oosterhof et al., 2018 - Colony-Stimulating Factor 1 Receptor (CSF1R) Regulates Microglia Density and Distribution, but Not Microglia Differentiation In Vivo

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

The Response to Neuronal Cell Death of csf1r Mutant Microglia Depends More on Recruitment Than on Proliferation

(A and B) We used our previously described conditional neuronal ablation model (van Ham et al., 2012, van Ham et al., 2014), in which treatment with metronidazole (MTZ) leads to selective ablation of neurons with transgenic expression of NTR (the nsfB gene encoding NTR). WT, csf1ra^−/−, and csf1ra^−/−;b^+/− larvae were treated with MTZ at 5 dpf for 16 hr and fixed for immunohistochemistry (whole-mount) at 6 dpf (A) and 7 dpf (B). Immunostaining was performed for dividing (Pcna⁺) microglia (L-plastin+), and the entire forebrain (dotted lines) was imaged and quantified. Scale bars, 40 μm. Group sizes were at least n = 10 zebrafish larvae (A) and at least n = 4 (B). Error bars indicate SD. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗p < 0.0001 (one-way ANOVA, Bonferroni multiple testing correction).

Expression Data

Antibody:	Ab2-lcp1
Fish:	c264Tg; gl22Tg; zf279Et csf1ra^j4e1/j4e1; c264Tg; gl22Tg; zf279Et csf1ra^j4e1/j4e1; csf1rb^re01/+; c264Tg; gl22Tg; zf279Et
Condition:	chemical ablation: neuron, chemical treatment by environment: metronidazole
Anatomical Term:	forebrain microglial cell
Stage Range:	Day 6 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	csf1ra^j4e1/j4e1; c264Tg; gl22Tg; zf279Et csf1ra^j4e1/j4e1; csf1rb^re01/+; c264Tg; gl22Tg; zf279Et
Condition:	chemical ablation: neuron, chemical treatment by environment: metronidazole
Observed In:	microglial cell microglial cell cell population proliferation
Stage:	Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.