Fig. 4

Cxcr2 Inhibitor SB225002 Blocks Neutrophil Reverse Migration

(A) Schematic of photoconversion of DMSO- or SB225002-treated larvae. Tg(mpx:Dendra) were wounded with tail transection at 3 dpf. DMSO or SB225002 was applied at 2 hpw. Photoconversion of neutrophils within the wounded fin (white dotted box in D) was performed at 3 hpw, and neutrophil reverse migration was assessed at 6 hpw.

(B) Sudan black staining at 6 hpw of WT larvae treated with DMSO or Cxcr2 inhibitor SB225002. Larvae were wounded at 3 dpf and treated with drug or vehicle control at 2 hpw.

(C) Quantification of neutrophils caudal to the notochord at 6 hpw (n = 20 DMSO larvae and 18 SB225002).

(D) Neutrophils pre- and post-photoconversion at 3 hpw and at 6 hpw were imaged in DMSO- or SB225002-treated larvae.

(E) Quantification of photoconverted neutrophils in the wound microenvironment (magenta cells in dotted boxes, D). SB225002-treated larvae contained a higher level of photoconverted cells within the tail fin at 3 and 6 hpw (n = 21 larvae, each).

(F) Percent of photoconverted cells that remained at the wound at 6 hpw was calculated. SB225002-treated neutrophils remain at the wound at a higher frequency than in DMSO control. ^∗p < 0.05.

Error bars represent SE.