FIGURE

Fig. 6

ID

ZDB-FIG-180907-10

Publication

Powell et al., 2017 - Chemokine Signaling and the Regulation of Bidirectional Leukocyte Migration in Interstitial Tissues

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Fig. 6

Cxcl8a Is Required for Neutrophil Reverse Migration from the Wound

(A) Average number of neutrophils at the wound is compared in WT, cxcr1−/−, cxcr2−/−, and cxcl8a−/− larvae (n = 13–32 larvae per time point/condition).

(B) Sudan black staining of neutrophils at 3 and 6 hpw in WT and cxcl8a−/−.

(C) Quantification of neutrophils at the wound shows more neutrophils at 6 hpw in cxcl8a−/− compared to heterozygote siblings (n = 74 WT and 75 mutants [3 hpw] and 91 WT and 70 mutants [6 hpw]).

(D) Representative tracks of neutrophils at the wound in cxcl8a+/− and cxcl8a−/− larvae.

(E) Neutrophil duration at the wound is greater in cxcl8a−/− than in heterozygotes (n = 17 het and 15 mutant larvae).

(F) Meandering index is decreased in cxcl8a−/− compared to het siblings. ^∗p < 0.05.

Error bars represent SE.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cxcr2^uwm35/uwm35
Condition:	transection: caudal fin
Observed In:	neutrophil migration regenerating fin neutrophil
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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