FIGURE

Fig. S3

ID

ZDB-FIG-180907-13

Publication

Powell et al., 2017 - Chemokine Signaling and the Regulation of Bidirectional Leukocyte Migration in Interstitial Tissues

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Fig. S3

Cxcr2 mutant sequence and rescue, related to Figure 1

(A) Amino acid sequence of Cxcr2 TALEN-generated mutation. Red arrow indicates mutated residue. (B) Initial recruitment of neutrophils to the wound is normal in Cxcr2-/- larvae (n= 49, 43). (C) Representative images of whole WT and Cxcr2-/- larvae at 3 dpf expressing Tg(mpx:Dendra) to visualize neutrophils. (D) Sudan black staining post-wound of WT and Cxcr2-/- larvae injected with cxcr2 mRNA. (E) Quantification of neutrophils at the wound shows cxcr2 mRNA rescues neutrophil resolution in Cxcr2-/- (n= 3hpw 24, 24, 24, 27; 6hpw 30, 33, 29, 28). (F) Velocity and (G) distance migrated of randomly migrating neutrophils is not significantly different in Cxcr2-/- compared to WT (tracks not shown, n= 25, 24). (H) Quantification of new neutrophils recruited to the wound after photo-conversion in Cxcr2-/- is greater at 6 hpw than WT (n= 25, 30). *p<0.05, n.s.= not significant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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