FIGURE

Fig. 5

ID

ZDB-FIG-180821-17

Publication

McMillan et al., 2018 - A regulatory pathway involving retinoic acid and calcineurin demarcates and maintains joint cells and osteoblasts in the fin regenerate

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Fig. 5

In evx1^−/− loss-of-function mutants, expression of hoxa13a but not pthlha persists in presumptive joint cells. (A) Wild-type fins possess joints (white arrowheads). (B,C) ISH on longitudinal cryosections of 4 dpa wild-type regenerates reveals hoxa13a (B) and pthlha (C) expression in joint cells (blue arrowheads). (D) In evx1^−/− mutants joints are lost. (E,F) Longitudinal cryosections of 4 dpa evx1^−/− mutant regenerates indicate hoxa13a (E) is expressed in presumptive joint cells but pthlha is not (F). Numbers in each panel represent the number of sections with the expression pattern over the total number of sections analyzed. Scale bars: 200 μm (in A for A,D); 50 μm (in B for B,C,E,F).

Expression Data

Genes:	hoxa13a pthlha
Fish:	WT evx1^i232/i232
Condition:	amputation: caudal fin
Anatomical Term:	regenerating fin joint
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	evx1^i232/i232
Condition:	amputation: caudal fin
Observed In:	regenerating fin joint
Stage:	Adult

Phenotype Detail

Acknowledgments

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