FIGURE

Fig. 4

ID

ZDB-FIG-180821-16

Publication

McMillan et al., 2018 - A regulatory pathway involving retinoic acid and calcineurin demarcates and maintains joint cells and osteoblasts in the fin regenerate

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Fig. 4

Sequential activation of hoxa13a, evx1 and pthlha expression in the presumptive joint cells. Double FISH and DAPI counterstains on longitudinal cryosections of 4?dpa fin regenerates illustrate three distinct patterns of expression. (A-C?) Patterns I and II are observed in rays with presumptive joint cells (arrowheads). (A?-C?) Pattern III is observed in rays without presumptive joint cells. (A-C?) In joint-forming cells (yellow arrows), the three markers are co-expressed in the joint-forming cells. (A,A?,C,C?) In the presumptive joint cells (green or yellow arrowheads), hoxa13a is expressed alone (Pattern I, A,C) or co-expressed with evx1 (Pattern II, A?) or pthlha (Pattern II, C?). (B?,C?) pthlha is also co-expressed with evx1 (B?). (B,B?) evx1 is either expressed alone (B) or co-expressed with pthlha (B?). Numbers in each panel represent the number of sections with the expression pattern over the total number of sections analyzed. Scale bar: 50??m (in A for A-C?).

Expression Data

Genes:	evx1 hoxa13a pthlha
Fish:	WT
Condition:	amputation: caudal fin
Anatomical Term:	regenerating fin joint
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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