FIGURE

Fig. S3

ID

ZDB-FIG-180803-20

Publication

Takayama et al., 2018 - Leucine/glutamine and v-ATPase/lysosomal acidification via mTORC1 activation are required for position-dependent regeneration

Other Figures

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Fig. S3

Lysosomal acidification in NH4Cl- and CQ-treated fins.

(A) Experimental scheme. A red two-headed-arrows indicates LysoTracker treatment, which was applied 15 min before observation. (B-E) Images of bright-field and fluorescence microscopy, and quantification of LysoTracker fluorescence intensities in control, NH₄Cl-, or CQ-treated fins at 3 hpa (n = 8). Black boxed areas in B-D are enlarged in B’-D’, respectively. The LysoTracker fluorescence intensities in red boxed areas were measured (B-D’). Representative images (B’-D’) used for quantification are shown in E. White dashed lines indicate the amputation planes. Scale bars: 1 mm (B-D) and 500µm (B’-D’). ***p < 0.001 by Student’s t test. Error bars represent the standard error.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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