FIGURE

Fig. S8

ID

ZDB-FIG-180702-9

Publication

Hocking et al., 2018 - Morphogenetic defects underlie Superior Coloboma, a newly identified closure disorder of the dorsal eye

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Fig. S8

Retinoic acid signaling and the superior ocular sulcus.

(A) Lateral views of eyes from 28 hpf zebrafish embryos that are gdf6a^+/+, gdf6a ^+/-, or gdf6a^-/- and have been processed for in situ hybridization. The top two rows show expression of the retinoic acid-synthesis genes cyp1b1 and aldha1a2. The bottom row shows expression of GFP in transgenic zebrafish carrying a reporter for RA signaling [Tg(12xRARE:GFP)] and are also gdf6a^+/+, gdf6a ^+/-, or gdf6a^-/-. Note reduced RA signaling in the superior retina of gdf6a ^+/- and gdf6a^-/- embryos. (B) Graph showing no effect of retinoic acid treatment on SOS closure. Embryos from gdf6a^+/- incrosses were grown from 10 hpf in control media, 5 nM retinoic acid, or 10 nM retinoic acid, and assessed at 28 hpf for an open SOS. (C) Graph showing no effect of the cyp1b1 mutation on SOS closure in gdf6a heterozygotes. gdf6a^+/-;cyp1b^+/- fish were crossed to wildtype or cyp1b^-/- fish and the percentage of embryos with an open SOS was assessed at 28 hpf. n = number of embryos, N = 2 (B) or 3 experiments (C). Data are means ± SEM. ns, not significant

Expression Data

Genes:	aldh1a2 cyp1b1
Fish:	WT gdf6a^s327/s327 gdf6a^s327/+
Anatomical Terms:	eye eye dorsal region eye ventral region
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gdf6a^s327/s327 gdf6a^s327/+
Observed In:	eye dorsal region
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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