FIGURE

Fig. S7

ID

ZDB-FIG-180702-8

Publication

Hocking et al., 2018 - Morphogenetic defects underlie Superior Coloboma, a newly identified closure disorder of the dorsal eye

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Fig. S7

Interaction between Hedgehog and Bone Morphogenetic Protein signaling in formation of the dorsal radial vessel.

Maximum projection confocal images of 34 hpf eyes from Tg(kdrl:eGFP);gdf6a^+/+ and Tg(kdrl:eGFP);gdf6a^-/- zebrafish embryos following treatment with control solution or 10 μM cyclopamine from 10 hpf. Blood vessels fluoresce green and the eye is outlined by dotted lines. DRV is indicated by arrow. Ectopic connection between superficial and hyaloid vasculatures indicated by arrowhead. Top row, right two panels are two examples of vessel overgrowth phenotype in cyclopamine-treated wildtype embryos. Bottom row, middle and right panels show the eyes of cyclopamine-treated gdf6a^-/- embryos that either failed to form a DRV (n = 3/6 embryos) or grew a simple DRV that did not make an ectopic connection to the hyaloid vasculature (n = 3/6 embryos), respectively. hv, hyaloid vasculature. Scale bar is 50 μm.

Expression Data

Gene:	GFP
Fish:	la116Tg gdf6a^s327/s327; la116Tg
Condition:	chemical treatment by environment: Cyclopamine
Anatomical Terms:	eye eye ventral region hyaloid vessel ocular blood vessel
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	la116Tg gdf6a^s327/s327; la116Tg
Condition:	chemical treatment by environment: Cyclopamine
Observed In:	superior ocular sulcus blood vessel
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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