FIGURE

Fig. 3

ID

ZDB-FIG-180702-2

Publication

Hocking et al., 2018 - Morphogenetic defects underlie Superior Coloboma, a newly identified closure disorder of the dorsal eye

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Fig. 3

The role of BMPR1 signaling in closure of the superior ocular sulcus.

(A-B) Effect of Bmpr1 antagonist DMH1 on SOS closure. Lateral view DIC images of eyes from live embryos (first row) and single optical slices of eyes processed for anti-Laminin immunofluorescence (second row) following exposure to control media or 0.02 μM DMH1, starting at either 10 or 18 hpf (A). SOS is marked by red asterisk. Quantification of delayed sulcus closure in DMH1-treated embryos (B). N = 3 experiments, n = 89 or 90 embryos for each condition. Data are means ± SEM. Statistics is a one-way ANOVA for each time series with Tukey's post-hoc test: **P<0.01. (C) Injection of caBMPR1A mRNA into one-cell stage zebrafish embryos caused expansion of eve1 gene expression into a circular ring in whole embryos at 50% epiboly (5.3 hpf). Significantly fewer embryos exhibited circular eve1 expression when injected with R471H-caBMPR1A. N = 3 experiments. Data are means ± SEM. Statistics is a two-tailed t test: *P<0.05. Scale bars are 50 μm.

Expression Data

Gene:	eve1
Fish:	WT
Anatomical Term:	margin
Stage:	50%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment by environment: DMH1
Observed In:	eye superior ocular sulcus
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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