FIGURE

Fig. 3

ID

ZDB-FIG-180627-3

Publication

Prabhudesai et al., 2018 - Cystathionine β-Synthase Is Necessary for Axis Development in Vivo.

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Fig. 3

Dose-dependent effect of cbsb morpholinos at 30 hpf. Phase contrast images of 30 hpf embryos injected with 4 ng of control (Con) MO (A); 1 ng cbsb splice1 (CBSB-S1) MO (B); 2 ng (CBSB-S1) (C) and 4 ng (CBSB-S1) (D) are shown. Arrow shows brain edema. Panels (E,F) quantifies the number of CBSA-S1 MO-injected embryos displaying bent axis, and brain edema (black arrows) compared to ConMO-injected embryos. n = 186 surviving embryos for CBSB-S1 MO and n = 272 surviving embryos for ConMO, which were a total of four experiments ****P < 0.0001 for both bent axis phenotype and brain edema phenotype. (G) Percentage survival observed over a 5 day period for three different doses of CBSB-S1-injected (1 ng, n = 70; 2 ng, n = 70; 4 ng, n = 70) and ConMO-injected embryos (4 ng, n = 70), n = 6 time points, **P < 0.01 for comparison between ConMO and each of the respective CBSB-S1 MO doses. Scale bars indicate 0.25 mm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-cbsb WT + MO3-cbsb WT + MO4-cbsb
Knockdown Reagents:	MO2-cbsb MO3-cbsb MO4-cbsb
Observed In:	head trunk whole organism whole organism anterior-posterior axis
Stage Range:	Prim-15 to Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol