FIGURE

Fig. 5

ID

ZDB-FIG-180608-107

Publication

Barske et al., 2018 - Essential Role of Nr2f Nuclear Receptors in Patterning the Vertebrate Upper Jaw

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Fig. 5

Edn1 Inhibits Nr2f Expression in the Mandibular Prominence

(A) edn1 is expressed comparably in the ventral ectoderm of wild-type controls and mutants at 20 hpf (arrowheads) and 30 hpf (magenta).

(B) Expression of the Edn1 receptors is reduced (ednraa) or largely unaffected (ednrab) in Nr2f mutants. Arch NCCs are labeled with dlx2a (green), and nr2f1b genotypes are indicated.

(C) In edn1 mutants, expression of all four Nr2f genes (magenta) ectopically increases to varying degrees in the mandibular domain (white arrows). Misexpression of edn1 induced by a heat shockof hsp70l:Gal4; UAS:Edn1 embryos from 20 to 24 hpf reduced Nr2f expression throughout the arches. Dashed lines reflect arch boundaries. Images are maximum-intensity projections. Merged images with dlx2a arch NCC staining are presented in Figure S5.

Scale bars, (A, top) 100 μm, (A, bottom, B, C) 20 μm.

Expression Data

Genes:	dlx2a edn1 ednraa ednrab nr2f1a nr2f1b nr2f2 nr2f5
Fish:	TU edn1^{tf216b/tf216b} (TU) el249Tg; kca4Tg (TU) nr2f2^el506/el506; nr2f5^el611/el611 (TU) nr2f1b^el523/+; nr2f2^el506/el506; nr2f5^el611/el611 (TU) nr2f1b^el523/el523; nr2f2^el506/el506; nr2f5^el611/el611 (TU)
Condition:	heat shock
Anatomical Terms:	mandibular arch skeleton non neural ectoderm pharyngeal arch 1 pharyngeal arch 1 neural crest cell pharyngeal arch 2 pharyngeal arch 2 neural crest cell
Stage Range:	20-25 somites to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	edn1^{tf216b/tf216b} (TU) el249Tg; kca4Tg (TU) nr2f1b^el523/+; nr2f2^el506/el506; nr2f5^el611/el611 (TU)
Condition:	heat shock
Observed In:	mandibular arch skeleton pharyngeal arch 1 pharyngeal arch 2
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 44(3), Barske, L., Rataud, P., Behizad, K., Del Rio, L., Cox, S.G., Crump, J.G., Essential Role of Nr2f Nuclear Receptors in Patterning the Vertebrate Upper Jaw, 337-347.e5, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell