FIGURE

Fig. 1

ID

ZDB-FIG-180515-1

Publication

Gagnon et al., 2017 - The primary role of zebrafish nanog is in extra-embryonic tissue.

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Fig. 1

Generation and phenotype of MZnanog mutants. (A) Top: TALENs were used to generate a 7 bp deletion within the first exon of nanog. Exons, introns, the open reading frame (ORF) and the homeobox domain are indicated. Bottom: The predicted mutant protein sequence, with intact amino acids (40 of 384 total) in normal font and frameshifted amino acids in bold font. Asterisk indicates a premature stop codon. (B) In situ hybridization for nanog expression in wild-type and MZnanog embryos at sphere stage. (C) Wild-type, Mnanog and MZnanog embryos imaged at 1.25?hpf (8-cell stage), 4?hpf (sphere stage) and 6.5?hpf (shield stage in wild type). Epiboly defects in Mnanog and MZnanog are apparent at 6.5?hpf. (D) MZnanog embryos are shown at 8?hpf and at 24?hpf, either uninjected or injected with 5?pg nanog mRNA at the 1-cell stage. In B and D, the number of embryos exhibiting the illustrated phenotype is shown in the bottom-right corner of each image as a proportion of total embryos examined.

Expression Data

Gene:	nanog
Fish:	WT nanog^a166/a166
Anatomical Term:	whole organism
Stage:	Sphere

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nanog^a166/a166 nanog^a166/a166 nanog^a166/+
Observed In:	epiboly whole organism
Stage Range:	Sphere to Shield

Phenotype Detail

Acknowledgments

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