FIGURE

Fig. 6

ID

ZDB-FIG-180417-41

Publication

Shi et al., 2017 - The Retinol Binding Protein Receptor 2 (Rbpr2) is required for Photoreceptor Outer Segment Morphogenesis and Visual Function in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Immunohistochemical analysis of rod and cone photoreceptors in WT and rbpr2 ^musc97 mutant zebrafish. Rod photoreceptor outer segments were identified with 1D4 antibody specific for rhodopsin (green, Rho, panels A–D). Cone photoreceptors outer segments were identified with PNA-488 (red, PNA, panels E–H) and Red/Green Opsin antibody (green, R/G Cone opsin, panels K–N) all at 5.5 dpf. Opsin mislocalization was observed in rbpr2 ^musc97 mutants (indicated by white arrows in D,H and N). Severe loss of rod and cone pigment proteins was evident in the rbpr2 ^musc97 mutant zebrafish (B,D,F,H,L and N). Quantification of photoreceptor outer segment length at 5.5 dpf is provided for rods (I) and cones (J). TUNEL staining for apoptosis in WT (O) and rbpr2 ^musc97 mutant (P) zebrafish retinas at 6 dpf. TUNEL positive cells/apoptotic nuclei stain green. Scale bars: 100 μm (A,B,E,F,K,L,O and P); 25 μm (C,D,G,H,M and N).

Expression Data

Antibodies:	Ab1-opn1lw Ab3-rho
Fish:	AB/TL stra6l^muz97/muz97
Anatomical Terms:	retinal cone cell photoreceptor outer segment retinal rod cell photoreceptor outer segment
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	stra6l^muz97/muz97
Observed In:	retina retinal cone cell photoreceptor outer segment retinal outer nuclear layer apoptotic process retinal rod cell photoreceptor outer segment
Stage Range:	Day 5 to Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.