FIGURE

Fig. 4

ID

ZDB-FIG-180417-39

Publication

Shi et al., 2017 - The Retinol Binding Protein Receptor 2 (Rbpr2) is required for Photoreceptor Outer Segment Morphogenesis and Visual Function in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Rbpr2 ^musc97 mutants show eye and systemic phenotypes consistent with retinoid deficiency. Lateral view of representative WT (panels A,C) and rbpr2 homozygous mutant (rbpr2 ^musc97 mut, panels C,D) zebrafish at 5.5 dpf. Rbpr2 ^musc97 mutants showed gross defects, which included: *hydrocephaly; **smaller eyes, ***pericardial edema and ****slight tail curvature. Scale bars = 0.326 mm (A,B) and 0.103 mm (C,D). (E) qPCR analysis of rbpr2 mRNA expression from WT and rbpr2 ^musc97 mutant zebrafish larvae at 5.5 dpf. (F) Injection of WT zebrafish rbpr2 mRNA or dose specific treatment with all-trans retinoic acid (ATRA) rescues the rbpr2 ^musc97 mutant phenotype. Images obtained at 5 dpf. Rescue experiments of rbpr2 ^musc97 mutants with either mRNA or ATRA were repeated twice as outlined in methods. (G,H) Transverse sections of 5.5 dpf WT (panel G) and rbpr2 ^musc97 mutant (panel H) eyes. rbpr2 ^musc97 homozygous mutant eyes were smaller and show disruption of retinal lamination layers.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	stra6l^muz97/muz97
Observed In:	eye retina layer formation retinal inner nuclear layer retinal outer nuclear layer
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.