FIGURE

Fig. 5

ID

ZDB-FIG-180313-18

Publication

Yanyi et al., 2017 - Activation of the Nkx2.5-Calr-p53 signaling pathway by hyperglycemia induces cardiac remodeling and dysfunction in adult zebrafish.

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Fig. 5

High glucose levels induced alterations of the Nkx2.5?Calr?p53 signaling pathway and apoptosis. (A) Real-time PCR analysis of the expression of Nkx2.5, Calr and p53 in the wild-type and treated fish in week 32 (n=8 fish per group). (B) Western blot analysis of the expression of Nkx2.5, Calr and p53 in wild-type (WT) and treated fish in week 32 (n=8 fish, repeated three times). (C) Merged images of cardiomyocytes (CMs) co-stained with Mef2 (red) and TUNEL (green) in the wild-type group (a), CMs exposed to 20?mM glucose (b) and CMs exposed to 20?mM glucose together with 10??M pifithrin-? (c) (n=20 field, repeated five times). Arrows: TUNEL+/Mef2+ CMs. Scale bars: 0.1?mm. (D) Quantitation of apoptotic CMs to total number of CMs in vitro of the three groups: CMs without any treatment, with 20?mM glucose, or with 20?mM glucose plus 10??M pifithrin-? (n=20 field per group, repeated five times). (A,D) Bars represent means�s.e.m. **P<0.01 compared with the wild-type group. ^#P<0.05 compared with 20?mM glucose group.

Expression Data

Genes:	calr3a nkx2.5 tp53
Fish:	AB
Condition:	chemical treatment by environment: glucose
Anatomical Term:	heart
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Condition:	chemical treatment by environment: glucose
Observed In:	heart
Stage:	Adult

Phenotype Detail

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