FIGURE

Fig. 5

ID

ZDB-FIG-180209-4

Publication

Botthof et al., 2017 - Loss of the homologous recombination gene rad51 leads to Fanconi anemia-like symptoms in zebrafish.

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Fig. 5

The HSPC defects in rad51^sa23805 fish are rescued in a p53 mutant background. (A) Representative images of 4-dpf embryos resulting from in-crosses of p53^+/− rad51^+/− parents stained using a cmyb-specific probe. The total number of embryos used (all genotypes) n = 237 from four clutches. For information about all genotypes, see SI Appendix, Table S3. Arrows indicate HSPCs. (Magnification, 100×.) (B) Percentage of BrdU⁺ cells in the kidney at 4 mpf at 1 dpi. Two-sided Student’s t test, n_p53^+/+ _rad51^+/+ = 5, n_p53^−/− _rad51^−/− = 5. (C) Number of total cells per kidney at 4 mpf quantified using a hemocytometer. Analysis using one-way ANOVA [F(3, 43) = 10.45, P < 0.0001], individual P values shown in the figure are from Tukey’s post hoc test, n_p53^+/+ _rad51^+/+ = 16, n_p53^+/+ _rad51^−/− = 16, n_p53^−/− _rad51^+/+ = 6, n_p53^−/− _rad51^−/− = 8. Bars represent mean ± SEM in B and C; n.s., not significant.

Expression Data

Gene:	myb
Fish:	rad51^{sa23805/sa23805}; tp53^zdf1/zdf1
Anatomical Term:	hematopoietic stem cell
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rad51^{sa23805/sa23805}; tp53^zdf1/zdf1
Observed In:	blood island hematopoietic stem cell head kidney hematopoietic stem cell head kidney hematopoietic stem cell proliferation
Stage Range:	Day 4 to Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA