FIGURE

Fig. S7

ID

ZDB-FIG-180209-8

Publication

Botthof et al., 2017 - Loss of the homologous recombination gene rad51 leads to Fanconi anemia-like symptoms in zebrafish.

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Fig. S7

P53 co-mutation increases the incidence of tumor development in double mutant fish, but does not rescue their fertility or size. (A) Male and female p53-/- rad51-/- fish at 4 mpf. (B) H&E stained histological sections of 4 mpf p53-/- and p53-/- rad51-/- ovaries using a 10X objective. Oocytes of different maturation stages are apparent in both genotypes. Scale bar=100μm. (C) H&E stained histological sections of 4 mpf p53-/- and p53-/- rad51-/- testes using a 20X objective. Arrows indicate normal location of mature spermatozoa Scale bar=100μm. (D) Quantification of the size of wild type and p53-/- rad51-/- fish at 4 mpf. Twotailed Student’s t-test, P=.008, n_{p53+/+ rad51+/+}=27, n_{p53-/- rad51-/-}=9. (E) 5-month-old p53-/- rad51-/- fish with tumor behind the eye (top). Accompanying H&E stained histological sections of the tumor (bottom) using 2.5 and 63X objectives. Scale bar=500 μm and 10 μm respectively. (F) (i) and (ii) Tumors observed in 8 mpf female p53-/- rad51-/- fish in the body cavity around the ovarian tissue. Scale bar=500 μm and 10 μm respectively. Overall, 3/9 aged double mutants showed tumors.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rad51^{sa23805/sa23805}; tp53^zdf1/zdf1
Observed In:	sex differentiation whole organism
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA