ZFIN Image: Botthof et al., 2017, Fig. 5

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Figure Caption

Fig. 5

The HSPC defects in rad51^sa23805 fish are rescued in a p53 mutant background. (A) Representative images of 4-dpf embryos resulting from in-crosses of p53^+/− rad51^+/− parents stained using a cmyb-specific probe. The total number of embryos used (all genotypes) n = 237 from four clutches. For information about all genotypes, see SI Appendix, Table S3. Arrows indicate HSPCs. (Magnification, 100×.) (B) Percentage of BrdU⁺ cells in the kidney at 4 mpf at 1 dpi. Two-sided Student’s t test, n_p53^+/+ _rad51^+/+ = 5, n_p53^−/− _rad51^−/− = 5. (C) Number of total cells per kidney at 4 mpf quantified using a hemocytometer. Analysis using one-way ANOVA [F(3, 43) = 10.45, P < 0.0001], individual P values shown in the figure are from Tukey’s post hoc test, n_p53^+/+ _rad51^+/+ = 16, n_p53^+/+ _rad51^−/− = 16, n_p53^−/− _rad51^+/+ = 6, n_p53^−/− _rad51^−/− = 8. Bars represent mean ± SEM in B and C; n.s., not significant.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA