FIGURE

Fig. S4

ID

ZDB-FIG-180201-4

Publication

Gong et al., 2017 - Insm1a Regulates Motor Neuron Development in Zebrafish

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Fig. S4

Primary motor neuron developmental defects in the insm1a morphants. A. Confocal imaging analysis of primary motor neuron in control group and insm1a morpholino injected groups at 30 hpf and 48 hpf Tg(mnx1:GFP)^ml2. Caps in dash line are showed in diagrams. Scale bar = 50 μ m. B. Quantification of distance between adjacent motor neurons (μm) in control group and insm1a morpholino injection groups at 30 hpf (n=23 and 41 respectively) and 48 hpf (n=26 and 51 respectively). C and D. The length and branching number of Cap axons in control group and insm1a morphant groups at 30 hpf and 48 hpf. Asterisks above the bars are significantly different (P<0.05). Values with ** above the bars are significantly different (P<0.05). E. Abnormal Caps in insm1a knockdown zebrafish embryos were restored by co-injection of insm1a mRNA with the morpholino. F. Quantification of zebrafish embryos with abnormal Caps.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ml2Tg + MO5-insm1a
Knockdown Reagent:	MO5-insm1a
Observed In:	CaP motoneuron CaP motoneuron axon MiP motor neuron
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.