FIGURE

Fig. S3

ID
ZDB-FIG-180104-8
Publication
Djenoune et al., 2017 - The dual developmental origin of spinal cerebrospinal fluid-contacting neurons gives rise to distinct functional subtypes
Other Figures
All Figure Page
Back to All Figure Page
Fig. S3

DAB deposition in APEX2 expressing CSF-cNs enables EM contrast in the spinal cord.

(a) Scheme of a 2.5 dpf larva showing the regions displayed in b and c. Lateral view of the rostral (b) and caudal (c) spinal cord of 2.5 dpf Tg(pkd2l1:Gal4)icm10 larvae injected with (UAS:APEX2- TagRFP) and revealed by DAB. Note the DAB+ CSF-cNs (arrowheads). (d-e) Close-ups showing that DAB+ cells are CSF-cNs characterized by their peculiar apical extension. White line represents the ventral limit of the spinal cord. (f-g) Transverse section showing in the spinal cord (SC) the specificity of the DAB deposition in a ventral CSF-cN (arrowhead). NC= notochord. (g) Close-up of the dotted rectangle region in (f) showing the DAB signal (purple) in a ventral CSF-cN (arrowhead) below the central canal (cc). Scale bars: 100 μm (b, c), 50 μm (d-f) and 20 μm (g).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.