FIGURE

Fig. 4

ID

ZDB-FIG-171228-20

Publication

Lawir et al., 2017 - A missense mutation in zbtb17 blocks the earliest steps of T cell differentiation in zebrafish

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Fig. 4

Unperturbed haematopoietic development in zbtb17 mutants.

(A) Microphotographs of wild-type and zbtb17 mutant embryos after RNA in situ hybridization with the indicated gene-specific probes and at the indicated times of development (scl, 36 hpf; myb, 36 hpf; gata1, 24 hpf). Scale bar, 100 µm (B) Representative microphotographs of control and morphant embryos on the ikaros:eGFP transgenic background to visualize lymphoid progenitors in the thymus and the caudal haematopoietic tissue (CHT) at 63 hpf. (C,D) Thymus homing assay in control and morphant embryos. Summary statistics of cohorts of embryos (examples in (B)) for cell counts at 63 hpf.

Expression Data

Genes:	EGFP gata1a myb tal1
Fish:	WT zbtb17^{t20463/t20463} fr101Tg fr101Tg + MO1-socs3b fr101Tg + MO1-zbtb17 fr101Tg + MO3-socs1a
Knockdown Reagents:	MO1-socs3b MO1-zbtb17 MO3-socs1a
Anatomical Terms:	caudal hematopoietic tissue erythroid lineage cell hematopoietic stem cell thymus primordium lymphoid progenitor cell
Stage Range:	Prim-5 to Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zbtb17^{t20463/t20463} fr101Tg + MO1-zbtb17 fr101Tg + MO3-socs1a
Knockdown Reagents:	MO1-zbtb17 MO3-socs1a
Observed In:	erythroid lineage cell hematopoietic stem cell T cell migration thymus lymphoid progenitor cell
Stage Range:	Prim-5 to Pec-fin

Phenotype Detail

Acknowledgments

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