FIGURE

Fig. 4

ID

ZDB-FIG-171205-26

Publication

Poureetezadi et al., 2016 - Prostaglandin signaling regulates nephron segment patterning of renal progenitors during zebrafish kidney development

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Fig. 4

Prostaglandin signal inhibition results in an expansion of the distal early domain, which is rescued by the addition of exogenous dmPGE₂.

Embryos were treated with a (A) 1% DMSO control, the (B) Ptgs1 selective inhibitor SC-560 at 50 μM from 4 hpf to 24 hpf, microinjected with the ptgs1 MO alone, or microinjected with the ptgs1 MO and treated with dmPGE₂ from 4 hpf to 24 hpf. Embryos were treated with the (C) Ptgs2a selective inhibitor NS-398 at 50 μM from 4 hpf to 24 hpf, microinjected with the ptgs2a MO or microinjected with the ptgs2a MO and then treated with dmPGE₂ at 50 μM from 4 hpf to 24 hpf. (D) Embryos were microinjected with a combination of the ptgs1 MO and ptgs2a MO. Embryos were microinjected with (E) ptger2a MO1, ptger2a MO2, (F) ptger4a MO1 and ptger4a MO2. (A–E) WISH was used to stain for the DE (slc12a1), DL (slc12a3) (purple), and the somites (smyhc1) (red) at the 24 hpf stage. Black bars indicate segment gene expression domain. Red scale bar, 70 µm. (G) Summary depicting the nephron segments after inducing deficiency of prostaglandin synthesis or receptor activity.

Expression Data

Genes:	slc12a1 slc12a3
Fish:	TU TU + MO1-ptger2a TU + MO1-ptger4a TU + MO1-ptgs1 TU + MO2-ptger2a TU + MO2-ptger4a TU + MO1-ptger2a + MO1-ptgs1
Conditions:	chemical treatment by environment: 16,16-dimethylprostaglandin E2 chemical treatment by environment: NS-398 chemical treatment by environment: SC560
Knockdown Reagents:	MO1-ptger2a MO1-ptger4a MO1-ptgs1 MO2-ptger2a MO2-ptger4a
Anatomical Terms:	intermediate mesoderm pronephric distal early tubule pronephric distal late tubule
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU TU + MO1-ptger2a TU + MO1-ptger4a TU + MO1-ptgs1 TU + MO2-ptger2a TU + MO2-ptger4a TU + MO1-ptger2a + MO1-ptgs1
Conditions:	chemical treatment by environment: 16,16-dimethylprostaglandin E2 chemical treatment by environment: NS-398 chemical treatment by environment: SC560
Knockdown Reagents:	MO1-ptger2a MO1-ptger4a MO1-ptgs1 MO2-ptger2a MO2-ptger4a
Observed In:	pronephric distal early tubule pronephric distal late tubule
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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