FIGURE

Fig. 4

ID

ZDB-FIG-171201-27

Publication

Burg et al., 2016 - Internal epitope tagging informed by relative lack of sequence conservation

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Fig. 4

Validation of internally epitope tagged Tcf21 and Tb18.

(a–d) Internally epitope tagged Tcf21 is fully functional and complements corresponding frameshift mutations. Lateral (a,b) and ventral (c,d) views of 5 dpf zebrafish embryos, with cartilage stained with Alcian Blue. Embryos trans-heterozygous for V5-tcf21 and tcf21 frameshift allele (a,c), and trans-heterozygous for different frameshift alleles (b,d). (e–h) Internally V5 epitope tagged Tcf21 and Tbx18 proteins are detectable by anti-V5 antibodies. Comparison between protein expression as detected by immunohistochemistry using anti-V5 antibodies (e,g) and mRNA expression detected by whole mount in situ hybridization (f,h).

Expression Data

Genes:	tbx18 tcf21
Fish:	tbx18^tpl116Tg/+ tcf21^tpl118Tg/+
Anatomical Terms:	fin bud head pharyngeal arch
Stage Range:	Prim-5 to Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tbx18^tpl116Tg/+; tbx18^tpl117/+ tcf21^tpl118Tg/+; tcf21^tpl119/+ tcf21^tpl118Tg/+; tcf21^tpl120/+ tcf21^tpl119/+; tcf21^tpl120/+
Observed In:	pharyngeal arch cartilage
Stage:	Day 5

Phenotype Detail

Acknowledgments

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