|
Fig. 4
Validation of internally epitope tagged Tcf21 and Tb18.
(a–d) Internally epitope tagged Tcf21 is fully functional and complements corresponding frameshift mutations. Lateral (a,b) and ventral (c,d) views of 5 dpf zebrafish embryos, with cartilage stained with Alcian Blue. Embryos trans-heterozygous for V5-tcf21 and tcf21 frameshift allele (a,c), and trans-heterozygous for different frameshift alleles (b,d). (e–h) Internally V5 epitope tagged Tcf21 and Tbx18 proteins are detectable by anti-V5 antibodies. Comparison between protein expression as detected by immunohistochemistry using anti-V5 antibodies (e,g) and mRNA expression detected by whole mount in situ hybridization (f,h).