FIGURE

Fig. 2

ID

ZDB-FIG-170912-5

Publication

Xie et al., 2016 - S1pr2/Gα13 signaling regulates the migration of endocardial precursors by controlling endoderm convergence

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Fig. 2

In Gα₁₃-deficient embryos, endocardial precursors fail to migrate to the midline, instead moving toward the myocardial precursors. Epifluorescence time-lapse experiments were performed on control (A-H) and gna13a/b MO-injected (A′-H′) Tg(fli: EGFP/nkx2.5: Kaede) embryos (Supplementary movie 1) from 13 to 20 s Embryos were exposed to UV light at 10 s for 1 min to convert Kaede fluorescence from green to red (presented as magenta). Snapshots from movies showing the migration of endocardial (green, outlined by white dots in A, A′, white arrowheads in F-H) and myocardial (magenta, outlined by yellow dashed-lines in A, A′; yellow arrowheads in F′-H′) precursors at the stages indicated. White arrows: the leading region of migrating endocardial population. Asterisks: Non-myocardial cells labeled by Nkx2.5-Kaede. Dorsoanterior views. Scale bars: 100 µm.

Expression Data

Genes:	EGFP Kaede
Fish:	fb9Tg; y1Tg fb9Tg; y1Tg + MO1-gna13a + MO1-gna13b
Knockdown Reagents:	MO1-gna13a MO1-gna13b
Anatomical Terms:	endocardium myocardium
Stage Range:	10-13 somites to 20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fb9Tg; y1Tg + MO1-gna13a + MO1-gna13b
Knockdown Reagents:	MO1-gna13a MO1-gna13b
Observed In:	endocardial precursor endocardial precursor cardioblast migration to the midline involved in heart field formation endocardial progenitor cell migration to the midline involved in heart field formation endocardium cardioblast migration myocardial precursor cardioblast migration to the midline involved in heart field formation
Stage Range:	10-13 somites to 20-25 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 414, Xie, H., Ye, D., Sepich, D., Lin, F., S1pr2/Gα13 signaling regulates the migration of endocardial precursors by controlling endoderm convergence, 228-43, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.