Fig. 5

Fn assembly is disrupted in Gα₁₃ morphants. Whole-mount Fn immunostaining was performed in control and gna13a/b MO-injected Tg(fli: EGFP/sox17: memCherry) embryos raised at 25 °C at the stages indicated. (A-D) Fn assembly (magenta) in embryos at 10 s (A, C) Projections of XY views of confocal Z-stacks spanning the endocardial-cell populations (green), the endoderm (false-colored cyan). (A′, C′) Projections of a subset of z-plane in the region shown in boxes in A-F. (A′′, C′′) A single z-plane in A′,C′. (A′′′, C′′′) Images of XZ transverse sections of the regions indicated by white lines in A,C. (B-D) Relative Fn intensity over the endocardial-cell population, as determined by plot profile measurement on XY image of single z plane on A′′,C′′. Results from individual embryos are shown in gray and average intensity in control (B, blue) and gna13a/b MO-injected red (D, red) embryos. (E-H) Fn assembly (magenta) in embryos at 16 s (E, F) Projections of XY views of confocal Z-stacks spanning the endocardial-cell populations (green). (E′, F′) Fn assembly only (magenta) in E, F. (E′′, F′′) Images of XZ transverse sections of the regions indicated by white lines in E, F. (G) Fn fibril directions as determined by FibrilTool. (H) Localization of Fn fibrils in the dorsal, ventral or middle of the endodermal sheet. White and yellow arrowheads: enrichment of Fn fibrils in control and gna13a/b MO-injected embryos, respectively; yellow dashed lines: midline; D: dorsal; V: ventral; A: anterior, P: posterior; ML (mediolateral). Scale bars: 10 µm.