FIGURE

Fig. 7

ID

ZDB-FIG-170822-15

Publication

Kroeger et al., 2017 - The zebrafish kidney mutant zeppelin reveals that brca2/fancd1 is essential for pronephros development

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Fig. 7

Knockdown ofbrca2causes a reduction in expression of the podocyte markerwt1band an increase in the interrenal gland lineage. (A-C) At 24, 48 and 72 hpf, WT embryos display normal wt1b expression in podocytes, while microinjection of an MO targeting the brca2 start (MO1) or the splice acceptor of exon 21 (MO2) recapitulated the zep mutant phenotype, as observed by a reduction or complete abrogation of wt1b transcripts as assessed by WISH. Further, brca2 morphants had elevated (B) nr5a1a transcripts as assessed by WISH and (C) increased 3β-HSD chromogenic staining, which are quantified in (D,E), respectively. Asterisks (**) indicate p < 0.001 using student T-test. Embryos are shown in dorsal views, where black boxes demarcate the cervical region where podocytes and the interrenal gland develop.

Expression Data

Genes:	nr5a1a wt1b
Fish:	WT WT + MO2-brca2 WT + MO3-brca2
Knockdown Reagents:	MO2-brca2 MO3-brca2
Anatomical Terms:	interrenal primordium pronephric podocyte
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-brca2 WT + MO3-brca2
Knockdown Reagents:	MO2-brca2 MO3-brca2
Observed In:	interrenal primordium pronephric glomerulus development pronephric podocyte pronephros
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 428(1), Kroeger, P.T., Drummond, B.E., Miceli, R., McKernan, M., Gerlach, G.F., Marra, A.N., Fox, A., McCampbell, K.K., Leshchiner, I., Rodriguez-Mari, A., BreMiller, R., Thummel, R., Davidson, A.J., Postlethwait, J., Goessling, W., Wingert, R.A., The zebrafish kidney mutant zeppelin reveals that brca2/fancd1 is essential for pronephros development, 148-163, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.