FIGURE

Fig. 5

ID

ZDB-FIG-170822-13

Publication

Kroeger et al., 2017 - The zebrafish kidney mutant zeppelin reveals that brca2/fancd1 is essential for pronephros development

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Fig. 5

WGS identifies that thezepmutation is located in thebrca2gene. (A) SNP analysis localized the zep lesion to chromosome 15. Upon further analysis of the region of interest, (B) zep mutants were found to have a T to C mutation at the splice acceptor site between exons 20 and 21 of the brca2 gene. (C) Schematic of the brca2 gene with the zep and brca2^ZM_00057434 mutant alleles indicated. (D) brca2 expression is ubiquitous between the 12 ss and the 28 ss, though slightly elevated transcript levels are observed in the region of IM at 12 ss where the proximal pronephros and interrenal gland emerge (boxed area). Embryos shown in lateral views.

Expression Data

Gene:	brca2
Fish:	WT
Anatomical Terms:	intermediate mesoderm whole organism
Stage Range:	10-13 somites to 26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 428(1), Kroeger, P.T., Drummond, B.E., Miceli, R., McKernan, M., Gerlach, G.F., Marra, A.N., Fox, A., McCampbell, K.K., Leshchiner, I., Rodriguez-Mari, A., BreMiller, R., Thummel, R., Davidson, A.J., Postlethwait, J., Goessling, W., Wingert, R.A., The zebrafish kidney mutant zeppelin reveals that brca2/fancd1 is essential for pronephros development, 148-163, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.