ZFIN Image: Kroeger et al., 2017, Fig. 5

Image

Figure Caption

Fig. 5

WGS identifies that thezepmutation is located in thebrca2gene. (A) SNP analysis localized the zep lesion to chromosome 15. Upon further analysis of the region of interest, (B) zep mutants were found to have a T to C mutation at the splice acceptor site between exons 20 and 21 of the brca2 gene. (C) Schematic of the brca2 gene with the zep and brca2^ZM_00057434 mutant alleles indicated. (D) brca2 expression is ubiquitous between the 12 ss and the 28 ss, though slightly elevated transcript levels are observed in the region of IM at 12 ss where the proximal pronephros and interrenal gland emerge (boxed area). Embryos shown in lateral views.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 428(1), Kroeger, P.T., Drummond, B.E., Miceli, R., McKernan, M., Gerlach, G.F., Marra, A.N., Fox, A., McCampbell, K.K., Leshchiner, I., Rodriguez-Mari, A., BreMiller, R., Thummel, R., Davidson, A.J., Postlethwait, J., Goessling, W., Wingert, R.A., The zebrafish kidney mutant zeppelin reveals that brca2/fancd1 is essential for pronephros development, 148-163, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.