FIGURE

Fig. 5

ID

ZDB-FIG-170815-18

Publication

Lim et al., 2017 - Caveolae Protect Notochord Cells against Catastrophic Mechanical Failure during Development

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Fig. 5

Longitudinal Ultrastructure of 6-dpf cavin1b^−/− Notochords

(A–C) Electron micrographs of a notochord longitudinal section from a 6-dpf WT embryo. Magnified view in (B) shows tightly apposed neighboring notochordal cells. (C) depicts neighboring notochord cells and caveolae.

(D–F) Longitudinal electron micrographs of notochord regions in lesion-rich sites from 6-dpf cavin1b^−/− embryos, each image representing a different zebrafish. Note aberrantly shaped vacuoles and disrupted cell morphology. For correlative light microscopy image of lesion presented in (D), refer to Figure S4F.

(G and H) Longitudinal electron micrographs of cavin1b^−/− cell-cell septum between notochord cells showing a prominent reduction in caveola number.

Black arrow, notochord lesion; black arrowhead, caveola; white arrow, perinotochordal sheath. The scale bars represent (A, B, and D–F) 10 μm and (C, G, and H) 500 nm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cavin1b^uq7rp/uq7rp
Observed In:	notochord development notochord inner cell notochord inner cell caveola
Stage:	Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.