FIGURE

Fig. 5

ID

ZDB-FIG-170703-18

Publication

Nelson et al., 2017 - In Vivo Regulation of the Zebrafish Endoderm Progenitor Niche by T-Box Transcription Factors

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Fig. 5

Ta and Tbx16 Are Redundantly Required for Liver, Pancreas, and Gut Development

(A) GFP immunostaining in single and double morphant sox17:eGFP transgenic fish at 24 hpf and WISH analysis of broad endodermal organ marker foxa3, pancreas marker ins, and liver marker cp at 52–56 hpf in single and double morphants. l, liver; p, pancreas; s, stomach. Phenotypic classes as defined in (B) are indicated.

(B) Percentage of KD embryos in each phenotypic class identified by WISH. Compare with Figure S3D. Graphs represent 19–124 embryos per group.

(C) Genetic crosses and expected embryonic genotypes. Ta-enhanced (ta^−/−;tbx16^+/−) and tbx16-enhanced (ta^+/−;tbx16^−/−) genotypes are indicated.

(D) Phenotypic classes of embryos from genetic crosses indicated in (C) identified by WISH. Arrowheads indicate liver cp staining.

(E) Percentage of embryos in each phenotypic class from each genetic cross identified by WISH. Graphs represent 31–175 embryos per group. ^∗p ≤ 3 × 10⁻²; ^∗∗p ≤ 5 × 10⁻³; ^∗∗∗p ≤ 1 × 10⁻⁴; all other comparisons with wild-type are not significant (p = 0.1–1); Fisher’s exact test.

See also Figure S3.

Expression Data

Genes:	cp EGFP foxa3 ins
Fish:	AB AB + MO2-tbxta AB + MO3-tbx16 ha01Tg ha01Tg + MO2-tbxta ha01Tg + MO3-tbx16 AB + MO2-tbxta + MO3-tbx16 ha01Tg + MO2-tbxta + MO3-tbx16
Knockdown Reagents:	MO2-tbxta MO3-tbx16
Anatomical Terms:	digestive system endoderm gut intestinal bulb liver pancreas pharyngeal endoderm
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-tbxta AB + MO3-tbx16 ha01Tg + MO3-tbx16 AB + MO2-tbxta + MO3-tbx16 ha01Tg + MO2-tbxta + MO3-tbx16
Knockdown Reagents:	MO2-tbxta MO3-tbx16
Observed In:	determination of digestive tract left/right asymmetry determination of liver left/right asymmetry determination of pancreatic left/right asymmetry digestive system digestive tract development endoderm gut liver liver development pancreas pancreas development
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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