Fig. S3

Dorsoventral and anteroposterior patterning in gpx4b M and MZ mutants and morphants. (A) Expression of dorsal organizer markers in M^allele2 and MZ^allele2 mutants compared to WT embryos at 4.3 hpf as revealed by WISH. Upper panels are animal pole views with dorsal to the right. Arrows indicate the edges of the chd and gsc mRNA expression domains. Lower panels are dorsal views with animal pole up. (B) Quantification of the arc of marker Development 144: doi:10.1242/dev.144261: Supplementary information Development • Supplementary information expression shown in A. (C) Effects of gpx4b knockdown on the expression of the indicated dorsal organizer marker genes at 4.3 hpf as revealed by WISH. (D) Quantification of the arc of marker expression shown in C. (E,F) Expression of the indicated dorsoventral markers in M and MZ mutants at 6 hpf as revealed by WISH. (G) Quantification of the arc of each marker expression shown in E and F. (H) Expression of the indicated dorsoventral markers in gpx4b morphants compared to cMO injected embryos at 6 hpf as revealed by WISH. (I) Quantification of the arc of marker expression shown in H. (J) Expression of the indicated anteroposterior neural markers in gpx4b mutants at 12.5 hpf. The frequency of embryos with the indicated pattern is shown in the bottom right corner of each group. Total embryo numbers are given at the top of each bar in B, D, G, and I. Asterisk and arrow in panels indicate the edges of the indicated mRNA expression domains. t, top view with dorsal to the right; d, dorsal views with animal pole up; l, lateral view with dorsal to the right. Values are means±s.e.m. (n = 3). ***P < 0.001. n.s., not significant. Unpaired t test, Two-tailed. Scale bar = 200 μm.