FIGURE

Fig. 3

ID

ZDB-FIG-170602-12

Publication

Rong et al., 2017 - Glutathione peroxidase 4 inhibits Wnt/β-catenin signaling and regulates dorsal organizer formation in zebrafish embryos

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Fig. 3

Overexpression of gpx4b ventralizes zebrafish embryos and inhibits Wnt activity. (A) Classification of phenotypes at 24 hpf caused by forced expression of 600 pg gpx4b mRNA. (B) Percentage of embryos in each category as shown in A. Results are from three independent experiments and the total embryo numbers are given at the top. (C) Expression patterns of chd and gsc marker genes in embryos injected with 600 pg gfp or gpx4b mRNA at 4.3 hpf. The frequency of embryos with the indicated phenotypes is shown in the bottom right corner of each group. (D) Overexpression of Gpx4b inhibits endogenous Wnt signaling in vivo. Embryos were injected with TOPFlash reporter DNA with 600 pg gfp or gpx4b mRNA. (E) Gpx4b inhibits endogenous Wnt signaling in vitro. HEK293T cells were transfected with 600 ng GFP plasmid DNA or Gpx4b plasmid DNA together with TOPFlash reporter DNA. Values are means±s.e.m. (n=3). *P<0.05; **P<0.01. Unpaired t-test, two-tailed. Scale bars: 200 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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