The p53 signaling pathway is activated in fn10a mutants. (A) Gene expression in RPKM (Reads Per Kilobase of transcript per Million mapped reads) in mutants and WT-siblings. Yellow asterisks, p53 pathway genes; red circles, genes of the p53 pathway differentially expressed in mutants and their siblings; green circles, intron-retained genes in the p53 pathway; and blue circles, other RefSeq genes. (B) Genes of the p53 pathway were up-regulated and enriched with intron retention but few had both intron retention and increased mRNA levels in fn10a mutants. (C) Real-time PCR confirmed the transcriptional up-regulation of p53 pathway genes (n = 5). (D) Knockdown of p53 expression in fn10a mutants rescued neuronal apoptosis in the CNS and spinal cord. Note that ∼50% of p53MO-injected fn10a mutants had no TUNEL signals and ∼42% had fewer TUNEL signals. (E) Over-expression of upf1 mRNA partially reduced p53 expression in eftud2 morphants while it had no effect on p53 expression in wild-type embryos (n = 5).
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