FIGURE

Fig. 7

ID

ZDB-FIG-170428-32

Publication

El-Rass et al., 2017 - Disruption of pdgfra alters endocardial and myocardial fusion during zebrafish cardiac assembly.

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Fig. 7

Analysis of the developing heart suggested expression of Pdgfra-mRFP in the endocardium rather than the myocardium. Dissected hearts from +/+ and +/− GBT1300 siblings carrying either a Tg(cmlc2:EGFP) or Tg(flk1:EGFP) transgene were used to investigate the expression pattern of the fusion Pdgfra-mRFP at 30 hpf. Cmlc2 positive +/+ embryos show no Pdgfra-mRFP expression in the heart (A), while +/− embryos exhibit Pdgfra-mRFP expression internal to the myocardium (B). Flk positive +/+ embryos show no Pdgfra-mRFP expression in the heart (C), while +/− embryos suggested co-localization of Pdgfra-mRFP with the endocardium (D). Scale bar: 50 μm.

Expression Data

Genes:	EGFP mRFP1
Fish:	s843Tg twu34Tg pdgfra^smh1300Gt/+; s843Tg pdgfra^smh1300Gt/+; twu34Tg
Anatomical Terms:	cardiac muscle cell endocardium
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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