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Fig. 7
Analysis of the developing heart suggested expression of Pdgfra-mRFP in the endocardium rather than the myocardium. Dissected hearts from +/+ and +/− GBT1300 siblings carrying either a Tg(cmlc2:EGFP) or Tg(flk1:EGFP) transgene were used to investigate the expression pattern of the fusion Pdgfra-mRFP at 30 hpf. Cmlc2 positive +/+ embryos show no Pdgfra-mRFP expression in the heart (A), while +/− embryos exhibit Pdgfra-mRFP expression internal to the myocardium (B). Flk positive +/+ embryos show no Pdgfra-mRFP expression in the heart (C), while +/− embryos suggested co-localization of Pdgfra-mRFP with the endocardium (D). Scale bar: 50 μm.